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Description
n/a
Immunogen
Fraction of bovine brain GABA-A receptor affinity purified against immobilized benzodiazepine Ro7-1986/1 (1988 de Blas et al J Neurosci PMID 2828565, 1988 Vitorica et al J Neurosci PMID 2828566). Target initially identified as a GABA-A receptor by: - rat brain immunocytochemistry staining pattern via light microscopy identical to that seen via ligand autoradiography with 3H-muscimol- immunoprecipitation of 3H-muscimol-binding activity of the purified receptor complex- recognition of ~57 kDa protein in immunoblots against crude rat brain membrane fractions Binding epitope identified to be within amino acids 26-40 (QSVNDPGNMSFVKET, extracellular N-terminus, 1992 Ewert et al Brain Res, PMID 1377081) of human GABA-A-R-Beta3 (also known as Gamma-aminobutyric acid receptor subunit beta-3, accession number P28472)Mouse: 100% identity (15/15 amino acids identical)Rat: 100% identity (15/15 amino acids identical)86% identity (13/15 amino acids identical, QSVNDPSNMSLVKET) with GABA-A-R-Beta266% identity (10/15 amino acids identical, HSTNEPSNMSYVKET) with GABA-A-R-Beta1Hybridoma kindly donated by Angel de Blas, University of Connecticut.This NeuroMab antibody is considered Restricted and is therefore not available for commercial re-distribution on a for-profit basis.