The general strategy of microbial glycan microarray analysis is similar to that of other types of microarray. The microbial glycans are either synthesized or isolated and purified from a broad range of microbes, and then undergo derivatization and printing in a microarray format. They are deposited on the NHS-activated glass slides over a wide range of concentrations to facilitate optimal detection of host-pathogen interactions. GBPs with putative pathogen recognition activity or sera are incubated with the microarray to examine potential interactions with microbial glycans. The detection is usually the fluorescence with a microarray scanner.