Recombinant RFP was synthesized with an amino terimal 6XHIS tag and was expressed in E.coli. A 3 step process was used to purify the protein: a partioning separation using t-butanol, followed by hydrophobic interaction chromatography (HIC), and lastly IMAC nickel chromatography. Analysis by SDS-PAGE resulted in a pattern consistent with purified RFP and was estimated to be greater than 90% pure.
Size
100 μg
Concentration
1.0 mg/mL
Applications
RFP is suitable as a control for polyclonal or monoclonal anti-RFP in immunological assays. The molecular weight of RFP (calculated) is 27 kDa, but in a gel it is expected to run at approximately 28-30 kDa.
Other Names
control protein, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583