The Human CD14 ELISA Assay kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for CD14 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD14 concentrations and unknowns are pipetted into these wells. During the first incubation, the CD14 Antigens, standards and zeros are bound this is followed by a separate incubation where the biotinylated monoclonal antibody specific for CD14 is added. Sample Types: serum, plasma, cell culture. Range: 6.87 - 220 ng/ml. Sensitivity: 6 ng/mL. Time: 3.75 hours ? The Eagle Biosciences Human CD14 ELISA Assay kit is to be used for the in-vitro quantitative determination of CD14 in serum, plasma, or cell lysates. The assay will recognize both natural and recombinant CD14. The Eagle Biosciences Human CD14 ELISA Assay kit is for Research Use Only.