Sample Type: Urine Intended Use: Enzyme immunoassay for the manual and automated quantitative determination of normetanephrine in human urine. Test Principle: The assay procedure follows the basic principle of competitive ELISA whereby there is competition between a biotinylated and a non-biotinylated antigen for a fixed number of antibody binding sites. The amount of biotinylated antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the free biotinylated antigen is removed by a washing step and the antibody bound biotinylated antigen is determined by use of anti-biotin alkaline phosphatase as marker and p-nitrophenyl phosphate as substrate. Quantification of unknowns is achieved by comparing the enzymatic activity of unknowns with a response curve prepared by using known standards. Kit Components: Assay Buffer, Concentrate (10X), 1x50ml Acylation Reagent, 1x2.25ml Indicator Buffer, 1x10ml Hydrolyzation Tu