The Quantikine Rat IL-1 alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-1 alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat IL-1 alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant rat IL-1 alpha accurately. Results obtained using natural rat IL-1 alpha showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IL-1 alpha. Range: 15.60 - 1000 pg/mL. Sensitivity: 4.12 pg/mL. Time: 4.5 hours