The Quantikine Canine IL-10 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure canine IL-10 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant canine IL-10 and antibodies raised against the recombinant protein. Results obtained for naturally occurring canine IL-10 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural canine IL-10. Range: 15.60 - 1000 pg/mL. Sensitivity: 3.8 pg/mL. Time: 4.5 hours