The Quantikine Canine IL-6 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure canine IL-6 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant canine IL-6 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural canine IL-6 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring canine IL-6. Range: 31.30 - 2000 pg/mL. Sensitivity: 11.8 pg/mL. Time: 4.5 hours