human, mouse, bovine, dog, macaque, chimpanzee, rat
Isotype
n/a
Format
immunoaffinity purified
Size
100 μg
Concentration
0.87 mg/mL
Applications
ELISA, Western Blot. This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kDa in size corresponding to PTEN-P1 protein by western blotting in the appropriate cell lysate or extract.
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Description
This affinity-purified antibody is directed against the phosphorylated form of human PTEN-P1 protein at the pS29 and pT32 residues. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human PTEN-P1 pS29/pT32 protein and the antibody is specific for the phosphorylated form of the proteins. Approximately 3% reactivity is observed against the non-phosphorylated form of the immunizing peptide. A BLAST analysis was used to suggest cross reactivity with PTEN proteins from mouse, bovine, dog, macaque, chimpanzee and rat sources based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 223-237 of human PTEN-P1 protein.
Other Names
rabbit anti-PTEN-P1 pS29 pT32 antibody, Phosphatase and tensin homolog 2, Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog, PTEN, MMAC1, TEP1