ELISA, Western Blot. This antibody is suitable for ELISA and western blotting. The antibody may be used for other applications, such as RIA, immunohistochemistry or immunoprecipitation, but specific reaction conditions have not been developed.
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Description
This protein A purified mouse monoclonal antibody reacts with a 30 kDa protein corresponding to human LEFTY. No reactivity occurs against 6X HIS tag. Significant sequence homology exists between the human and mouse forms of LEFTY. Cross reactivity is expected with mouse and rat forms of the protein. Reactivity with other sources has not been determined.
Immunogen
A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.
Ulloa, L. and Tabibzadeh, S. (2001) Lefty inhibits receptor-regulated Smad phosphorylation induced by the activated transforming growth factor-beta receptor. J Biol Chem, 276(24): 21397-404. Meno, R. et al: (1998) Lefty -1 is required for the left-right determination as a regulator of Lefty-2 and Nodal. Cell 94: 287. Tsukui, T., et al. (1999) Multiple left-right asymmetry defects in Shh-/- mutant mice unveil a convergence of the Shh and retinoic acid pathways in the control of Lefty-1. Proc. Natl. Acad. Sci USA 96: 11376-11381.