ELISA, Western Blot. This antiserum against Mouse Leptin has been tested for use in ELISA and immunoblotting. Reactivity in other immunoassays is unknown. Obesity associated leptin is the product of the OB gene and has been identified with Type II diabetes.This product has been assayed by immunoblot against tissue homogenates using HRP Goat-anti-Rabbit IgG [H&L] (code # 611-1302) and TMB as a substrate. A working dilution range of 1:200 to 1:400 is suggested for this application. This product has been assayed by ELISA against recombinant mouse leptin using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code # 611-1302) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:2,400 is suggested for this product.
Reviews / Ratings
If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY.
Description
This antiserum has been heated to 56°C for 30 minutes. The antibody will recognize recombinant and native 16 kDa leptin from mouse and human. Reactivity with leptin from other sources is unknown.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with recombinant mouse leptin 16,000 MW produced in E. coli.
Other Names
rabbit anti-Leptin, LEP antibody, Leptin Murine Obesity Homolog antibody, Leptin Precursor Obesity Factor antibody, OB antibody, Obese Protein antibody