ELISA, Immunohistochemistry, Western Blot. Anti-ALG6 antibody has been tested in ELISA, western blot, and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 58kDa in size corresponding to ALG6 protein by Western blotting in the appropriate cell lysate or extract.
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Description
ALG6 Antibody antiserum is directed against human ALG6. A 58 kDa band is detected when assayed by immunoblot. Cross reactivity is expected against human ALG6 protein based on a 100% homology of the amino acid sequence of the immunogen between rat, mouse, drosophila, and pongo abelii.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, corresponding to a region near the N-terminus of human ALG6 protein, conjugated to KLH.