ELISA, Western Blot. This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 76 kDa in size corresponding to PKC beta by western blotting in the appropriate cell lysate or extract. Lysates from U251or BxPC3 cells are suggested for western blotting.
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Description
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human PKC beta protein. A BLAST analysis was used to suggest cross-reactivity with PKC beta from human and monkey sources based on a 100% homology with the immunizing sequence. Expect partial cross reactivity to PKC beta from rabbit and bovine (93% homology) and rat and mouse (87% homology) sources. Cross-reactivity with PKC beta from other sources has not been determined.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Protein kinase C beta.
Other Names
rabbit anti-Protein kinase C beta antibody, PKC β, Protein kinase C beta type, PRKCB, PKCB, PRKCB1, PKC-beta, PKC-B