ELISA, Flow Cytometry, Immunohistochemistry, IF Microscopy, Immunoprecipitation, Western Blot. Anti-xCT Antibody has been tested in Western Blot, ELISA, Immunofluorescence, and flow cytometry. Expect a band at 55.4 kDa in western blot using appropriate lysates. Positive control used HeLa, NIH-3T3, A549, HCT-116, or PC3 whole cell lysates at 0.1-1.0μg/mL for WB and PC3 with MeOH at 10μg/mL in IF. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.
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Description
This affinity purified antibody is directed against human xCT. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human and orangutan, and 88% identity to mouse.
Immunogen
Anti-xCT antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human xCT conjugated to Keyhole Limpet Hemocyanin (KLH).
Other Names
rabbit anti-xCT antibody, SLC7A11, Cystine/glutamate transporter, Amino acid transport system xc-, Calcium channel blocker resistance protein CCBR1, Solute carrier family 7 member 11