FLISA, IF Microscopy, Western Blot. The emission spectra for this DyLight? conjugate match the principle output wavelengths of most common fluorescence instrumentation.
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Description
6X His Tag Antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
Immunogen
This 6X HIS antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Other Names
mouse anti-6X His Tag biotin conjugated Antibody, biotin conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody
Brandsma, M ; X Wang, H Diao, SE. Kohalmi1, AM Jevnikar and S. Ma (2009) A Proficient Approach to the Production of Therapeutic Glucagon-Like Peptide-1 (GLP-1) in Transgenic PlantsThe Open Biotechnology Journal, 3, 57-66 57 1874-0707/09