Avidin was prepared from chromatographically purified avidin isolated from egg white followed by extensive dialysis against the buffer stated above. Avidin was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin. No reaction was observed against anti-Streptavidin.
Size
10 mg
Concentration
10 mg/mL
Applications
ELISA, Chip, Gel Shift, Western Blot. Avidin is suitable for use as antigen, as a control or standard in assays, and most other immunological methods as well as enzyme conjugates and complexes; Southern blots and other methodologies related to DNA and RNA analysis; Western blots; and purification of proteins or other antigens with biotinylated antibodies or lectins by use of immobilized avidin.