Sequence: 5'-G A C G T^C-3' 3'-C^T G C A G-5' Supplied With: Restriction Enzyme Buffer A (R1625): (for 100% AatII digestion) 33mM Tris-acetate (pH 7.9), 10mM magnesium acetate, 66mM potassium acetate and 0.1mg/ml BSA. Incubate at 37°C. Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM potassium chloride, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM potassium chloride, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after a 160-fold overdigestion with AatII (10u/ug lambda DNA x 16 hours). Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with AatII more than 95% of the digested DNA fragments can be ligated at a 5'-termini concentration of 0.1uM. More than 95% of these sites can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonu