5'-G A C N^N N G T C-3' 3'-C T G N N^N C A G-5' Source: Pseudomonas syringae Lki1-pH124 Concentration: 10u/ul Unit Definition: One unit is defined as the amount of P9181-50 required to digest 1ug of lambda DNA-Smal fragments in 1 hour at 37°C in 50ul of assay buffer. Supplied as a liquid in 10mM Tris-HCl, pH 7.4, 50mM sodium chloride, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol. Supplied with R1625-01 Restriction Enzyme Buffer B, 10X: Supplied as a liquid in 10mM Tris-HCl pH 7.5, 10mM MgCl2, 0.1mg/ml BSA. Incubate at 37°C Supplied with R1625 Restriction Enzyme Buffer A, 10X: Supplied as a liquid in 10mM Tris-HCl pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.