Collagenase, Type I, Sterile Filtered, C. histolyticum
Description
Activity: ≥125 units/mg dry weight. Collagenases degrade native helical collagen fibrils.The substrate, collagen, is the major fibrous component of animal extracellular connective tissue: skin, tendon, blood vessels, bone, etc. Suitable for use in cell dissociation procedures. Activity: ≥125 units/mg dry weight. Caseinase: As reported Clostripain: As reported Tryptic: As reported Unit Definition: 1 unit releases 1umole of L-leucine equivalents from collagen in 5 hours at 37°C, pH 7.5. Absorbance (A280): As reported Collagenase nomenclature: Type 1: Containing average amounts of assayed activities (collagenase, caseinase, clostripain and tryptic activities). It is generally recommended for fat, adrenal and liver cells. Type 2: Containing greater clostripain activity. It is generally used for heart, bone, muscle, thyroid, cartilage and liver cells. Type 3: Containing low proteolytic activity. It is usually used for mammary and fetal cells. Type 4: Containing low tryptic activity. It is c