BAFF Receptor, Recombinant, Human (BAFF-R, BR3, BLyS Receptor 3)
Description
Purity ≥ 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. Recombinant Human extracellular domain BAFF receptor, produced in E.coli is a single, non-glycosylated polypeptide chain containing 63 amino acids fused with GST tag and having a molecular mass of 33600 Dalton. Recombinant BAFF-R is purified by proprietary chromatographic techniques. Solubility: It is recommended to reconstitute the lyophilized Recombinant Human BAFF-R in sterile 18Mω-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Dimers and aggregates: ≤ 1% as determined by silver-stained SDS-PAGE gel analysis. Biological Activity: Measured in a cell inhibition assay using B lymphocyte cell line. (The ED50 for this effect is typically 50~100ng/ml in the presence of 2.5ng/ml BAFF). Endotoxin: ≤ 0.1ng/ug (IEU/ug) of Human BAFF-R.