Purity ~95% (SDS-PAGE gel, HPLC). Human BAFF (for B cell activating factor belonging to the TNF family) is a newly discovered novel ligand of the TNF family. BAFF plays an important role as costimulator of B cell proliferation and function. Recombinant Human BAFF is a soluble 17kD protein containing 153 amino acid residues, comprising the TNF-like extra-celluar domain of BAFF. Source: Recombinant protein corresponding to human BAFF, expressed in E. coli. Biological Activity: Assay #1: Determine by the dose-dependent stimulation of IL-8 production by human PBMC. The expected ED50 for this effect is 5-10ng/ml. Note: Results may vary with PBMC donors. Assay #2: Determined by a mouse splenocyte survival assay. The expected ED50 for this effect is 0.5-2.0ug/ml Endotoxin: <0.1ng/ug of protein (<1EU/ug) AA Sequence: AVQGPEETVT QDCLQLIADS ETPTIQKGSY TFVPWLLSFK RGSALEEKEN KILVKETGYF FIYGQVLYTD KTYAMGHLIQ RKKVHVFGDE LSLVTLFRCI QNMPETLPNN SCYSAGIAKL EEGDELQLAI PRENAQISLD GDVTFFGALK LL
Size
5ug, 20ug
Concentration
n/a
Applications
n/a
Other Names
BAFF, Recombinant, Human, Animal Free (B Cell Activating Factor Belonging to the TNF Family, TNFSF13B, TNFSF20, TALL-1, BLys, THANK)