HSV type 1 grade 2 antigen is collected from both the cell and supernatant phases of tissue culture. Harvest of antigen is carried out by disrupting optimally infected monolayers into the tissue culture medium. Cellular debris is removed by centrifugation. . Herpes simplex virus (HSV) is a double stranded DNA, enveloped virus, 110nm to 120nm in diameter. The icosahedral viral capsid is assembled in the host cell nucleus followed by the virus acquiring its envelope by budding through the nuclear membrane at sites modified by the addition of viral protein. The HSV genome encodes over 50 polypeptides, including 9 glycoproteins (gB to gJ), at least 6 capsid proteins, viral protein kinase and DNA polymerase. There are two serotypes, HSV type 1 and HSV type 2 that can be differentiated on the basis of antigenicity of glycoprotein gG. The serotypes have approximately 40% homology leading to antigenic cross reactivity. Source: HSV, strain MacIntyre, from BSC-1 Applications: Suitable for use in for both IgG and IgM detection in assays which include EIA with polystyrene and latex solid phases. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inactivation: HSV antigen is inactivated using gamm