Purity ~90% (RP-HPLC, SDS-PAGE). HtrA2, also called Omi, is a mammalian serine protease at high temperatures and has a chaperone activity at low temperature. The full-length HtrA2 is synthesized as a precursor protein and then targeted to the mitochondria where it is matured by the removal of N-terminal 133 residues. Mature HtrA2 consists of a putative transmembrane domain; an inhibitor of apoptosis protein (IAP)-binding motif; a single C-terminal PDZ domain that mediates protein-protein interactions. Recently, HtrA2 has known to contribute both to caspase-dependent and caspase-independent cell death. Source: Recombinant protein corresponding to aa134-458 from human HTRA2, fused to his-tag, expressed in E. coli. Molecular Weight: ~32kD