The Quantikine Mouse Leptin Immunoassay is a 4.5 hour solid phase ELISA designed to measure soluble mouse leptin in cell culture supernates, serum, and plasma. It contains recombinant mouse leptin expressed in E. coli and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse leptin accurately. Results obtained measuring natural mouse leptin showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse leptin. Pregnant mouse serum from the second trimester of pregnancy through post-partum day 1 require acid/urea activation of mouse leptin. Range: 62.50 - 4000 pg/mL. Sensitivity: 22 pg/mL. Time: 4.5 hours