Purity: > 90% by SDS - PAGE Format: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 10% glycerol,1mM DTT . CYTH2, also known as Cytohesin-2 and ARNO, is an ARF-1 guanine nucleotide exchange factor (GEF). ARF (ADP ribosylation factor) proteins, a group within the RAS superfamily, are GTP-binding proteins central to the process of vesicle budding. This protein promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Also it promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane. Recombinant human CYTH2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.