Non-viable cell pellet was generated from cell line WM3282. WM3282 is a tumorigenic (VGP) primary melanoma cell line with competence for metastasis. These cells display pigmented, mesenchymal morphology in culture. This cell line contains V600K (Val600Lys) mutation at codon 600 in the BRAF gene. This mutation occurs within the activation segment of the kinase domain and causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. WM3282 cells produce xenograft tumors when injected into immunocompromised mice.
Size
20 mg
Concentration
20 million cells
Applications
Frozen non-viable cell pellets can be used for in vitro experiments such as Western blotting and other immunoassays, genomic DNA isolation, STR profiling, and RNA isolation.
Other Names
Human Melanoma, patient derived tumor, tumor models, skin cancer, xenograft