The Eagle Biosciences Mouse Adiponectin ELISA Assay Kit is a so-called Sandwich-Assay using two specific and high affinity antibodies. The Adiponectin in the samples binds to the first antibody coated on the microtiter plate. In the following step the second specific anti-Adiponectin-Antibody binds in turn to the immobilised Adiponectin. The second antibody is biotinylated and will be applied in a mixture with a Streptavidin-Peroxidase-Enzyme Conjugate. In the closing substrate reaction the turn of the colour will be catalysed quantitatively depending on the Adiponectin-level of the samples. Sample Types: serum, plasma. Range: 0.025 - 1 ng/mL. Sensitivity: 0.008 ng/mL. Time: 2.5 hours ? Adiponectin was described for the first time in the early 90th of the last century as an Endocrinology factor produced by adipocytes. Adiponectin is involved in regulation of energy- and fat metabolism. So its concentration in the circulation is said to reflect the risk of atherosclerosis and the degree of insulin resistance. Based on the high incidence of these diseases, adiponectin was and still is object of intensive research regarding the underlying biological mechanisms and regarding its value as biomarker. Beside different cell culture models and studies with human patients, mice and rats are suitable model organisms for basic research and pre-clinical studies.