This kit was based on sandwich enzyme-linked immune-sorbent assay technology. thrombin IIIwas pre-coated onto 96-well plates. And the Biotin- labeled thrombin III was used as detection antigen. The standards, test samples and Biotin- labeled thrombin III were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin Conjugate was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by H