Western Blot (WB), Immunofluorescence Microscopy (IF)
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Description
Purified anti-CD171 (L1CAM). L1CAM is a 200–220 kD transmembrane glycoprotein of the immunoglobulin (Ig) superfamily. It is composed of 6 Ig-like domains and 5 fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail. The members of the L1 family are L1-CAM (CD171), close homolog of L1-CAM (CHL1), neurofascin (NFASC) and NgCAM related cell adhesion molecule (NR-CAM). L1CAM can bind to itself (homophilic) or heterophilically to other neural cell adhesion molecules, integrins, CD24, neurocan and neuropilin-1. The cytoplasmic tail of L1CAM can interact with the cytoskeletal proteins ankyrin, actin, spectrin and ERM proteins. L1CAM was discovered as a cell adhesion molecule in the nervous system. During brain development, L1CAM is involved in neurite outgrowth and fasciculation, as well as adhesion and migration. In addition, L1CAM participates in myelination processes by mediating the elongation of Schwann cells along the axon and promotes neural cell survival. Mutations of the L1CAM gene cause severe recessive cerebral disorders like X-linked mental retardation and schizophrenia and frequently lead to neonatal death. L1CAM is overexpressed in many human cancers, such as ovarian and endometrial carcinoma, pancreatic cancer, melanoma and glioblastoma. L1CAM expression is usually associated with poor prognosis, an aggressive phenotype, and advanced tumor stages. Unlike in neural cells, L1CAM in the tumor setting rarely promotes static cell-cell adhesion, but induces a motile and invasive phenotype, supporting an aggressive tumor growth, metastasis and chemoresistance.
Immunogen
Full-length recombinant human CD171 produced in 293T cells.