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Description
In order to generate this mAb, the HLA.A2 heavy chain and human b2-m were produced in bacteria, purified and covalently bound to the immunodominant HLA.A2 restricted peptide 58-66 of the influenza virus matrix protein (M1p). Correct refolding was confirmed by staining specific CD8+ CTL clones with the biotinylated recombinant chimera that had been tetramerized with PE-streptavidin. Balb/c mice were immunized with the chimera and hybridomas were tested by a differential chimera-based ELISA containing M1-HLA.A2 versus HIVgag-HLA.A2 and empty HLA.A2 constructs. 405H1 mAb was found to be specific for M1-HLA.A2 with a Kd = 3,3.10-10 M. 405H1 represents a valuable tool for studying the processing and direct or cross-presentation on MHC-I molecule.