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Description
ITGAV/Integrin Alpha V/CD51 Antibody (clone NKI-M7 (former AMF7)) LS-C347518. Specificity: T-lymphocyte clones NKI-M7 detect a melanoma-associated proteoglycan (MW greater than 450-250 kD) and a molecular complex, which under reducing conditions consists of 4 compounds of 120, 95, 29 and 25 kD respectively. NKI-M7 reacts with cultured melanomas melanomas in frozen sections. The antigen detected by NKI-M7 is strongly associated with the adhesion and cytoplasmic spreading of melanoma cells to plastic surfaces and monolayers of vascular endothelial cells. NKI-M7 blocks fibronectin-induced chemotactic motility and chemokinesis of melanoma cells. In addition to their membrane localization, the antigens detected by NKI-M7are abundant in extracellular adhesion plaques deposited by cultured melanoma cells. NKI-M7 stains primary tumors heterogeneously, whereas metastases are homogeneously stained.
Immunogen
NKI-M7 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with cultured melanoma cells derived from a Human melanoma metastasis.