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Description
MCAM / CD146 Antibody (FITC) LS-C152896. Specificity: Reacts specifically with CD146 {MCAM, MUC18}. In blood and marrow P1H12 reacts only with endothelial cells, and has been used to detect circulating endothelial cells in human, dogs, rabbits and mouse samples. It positively stains normal, primary HUVEC and MVEC cultures and the endothelial cells of all vessels in normal frozen sections of human skin, intestine, ovary tonsil, lymph node, lung, and kidney. It does not stain carcinoma cell lines HT-29 and COLO205, and M21, the T cell lines Jurkat and HuT78, fibroblasts, HL-60 or CHO cells, or EBV-transformed B cell lines, although it expected that CD146 expression is present in many tumor lines. P1H12 does not stain normal monocytes, granulocytes, red cells, platelets, T cells or B cells from marrow or peripheral blood; nor does it detect marrow megakaryocytes or the megakaryoblast line HU3. The peripheral blood cells that do stain with P1H12 also are positive for both von Willebrand Factor (vWF) and thrombomodulin (the combined expression of which is limited to endothelium), and they stain for flt and flk. In recent testing, melanoma A2058, SKMEL.3 and A375. S2 are positive, while murine melanoma M3 is negative. One smooth muscle line (HISM) was positive and one negative (TIGHA-VSMC). Human blood lymphocytes are negative with or without prior stimulation