PRMT4 (CARM1) is a histone methyltransferase that exhibits methylation activity toward H3-R17. Intended Use: The PRMT4 Direct Activity Chemiluminescent BioAssay? kit is designed to measure PRMT4/CARM1 activity activity for screening and profiling applications. Test Principle: The key to the PRMT4 Chemiluminescent BioAssay? Kit is a highly specific antibody that recognizes methylated PRMT4 substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the strip plates are treated with an HRP-labeled secondary antibody followed by addition of an HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt Applications: Great for studying enzyme kinetics,