PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3. Intended Use: The PRMT5 Direct Activity Chemiluminescent BioAssay? Kit is designed to measure PRMT5 activity for screening and profiling applications. Test Principle: The key to the PRMT5 Chemiluminescent BioAssay? Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt Applications: Great for studying enzyme kinetics, screenin