PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3. Intended Use: Suitable for studying enzyme kinetics and HTS applications. Test Principle: The PRMT5 Chemiluminescent BioAssay? Kit is designed to measure PRMT5 activity for screening and profiling applications. The PRMT5 Chemiluminescent BioAssay? Kit comes in a convenient format, with a 384-well plate precoated with histone H4 peptide substrate, the antibody against methylated arginine3 residue of Histone H4, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified PRMT5 enzyme for 384 enzyme reactions. The key to the PRMT5 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, prima