Intended Use: Suitable for studying enzyme kinetics and HTS applications. Test Principle: The PRMT9 Homogeneous BioAssay? Kit is designed to measure PRMT9 activity for screening and profiling applications. PRMT9 is a histone methyltransferase that exhibits methylation activity toward an arginine residue of SAP145. The PRMT9 Homogeneous BioAssay? Kit comes in a convenient AlphaLISA format, with GST-tagged PRMT9 substrate, primary antibody, methylation assay buffer, and purified PRMT9 for 384 enzyme reactions. The key to the PRMT9 Homogeneous BioAssay? Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT9 enzyme is incubated with the substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts. Contraindications: Green and blue dyes that absorb light in the AlphaScreen signal