PRMT3 is a histone methyltransferase that exhibits methylation activity toward H4-R3. Intended Use: Suitable for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. Test Principle: The PRMT3 Homogeneous BioAssay? Kit is designed to measure PRMT3 activity for screening and profiling applications. PRMT3 is a histone methyltransferase that exhibits methylation activity toward H4-R3. The PRMT3 Homogeneous BioAssay? Kit comes in a convenient AlphaLISA format, with biotinylated histone H4 peptide substrate, primary antibody, methylation assay buffer, and purified PRMT3 for 384 enzyme reactions. The key to the PRMT3 Homogeneous BioAssay? Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT3 enzyme is incubated with the biotinylated substrate for one hour. Next, acceptor beads and pri