PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3. Intended Use: Suitable for studying enzyme kinetics and HTS applications. Test Principle: The PRMT5 Homogeneous BioAssay? Kit is designed to measure PRMT5 activity for screening and profiling applications. PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3. The PRMT5 Homogeneous BioAssay? Kit comes in a convenient AlphaLISA format, with biotinylated histone H4 peptide substrate, primary antibody, methylation assay buffer, and purified PRMT5 for 384 enzyme reactions. The key to the PRMT5 Homogeneous BioAssay? Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT5 enzyme is incubated with the biotinylated substrate for two hours. Next, acceptor beads and primary antibody are added, then donor beads, followed by read