PRMT1 is a histone methyltransferase that exhibits methylation activity toward H4-R3. Intended Use: Suitable for use in studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. Test Principle: The PRMT1 Homogeneous BioAssay? Kit is designed to measure PRMT1 activity for screening and profiling applications. PRMT1 is a histone methyltransferase that exhibits methylation activity toward H4-R3. The PRMT1 Homogeneous BioAssay? Kit comes in a convenient AlphaLISA format, with a 384-well plate, biotinylated histone H4 peptide substrate, primary antibody, methylation assay buffer, and purified PRMT1 for 384 enzyme reactions. The key to the PRMT1 Homogeneous BioAssay? Kit is a highly specific antibody that recognizes methylated histone substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT1 enzyme is incubated with the biotinylated substrate for one h