this line was originally established by M. Rollinghoff and N.L. Warner from a fibrosarcoma induced by subcutaneous injections of 3-methylcholanthrene; after pretreatment with actinomycin D, the cells are highly sensitive to human cytotoxic monocytes, to human tumor necrosis factor and to lymphotoxin; they can be used as target cells for short term chromium release cytotoxicity assays; tested and found negative for ectromelia virus (mousepox)Tumorigenecity:Isoenzyme:Histopathology:fibrosarcomaSubculture:Split ratio:Media change:Reverse transcritase:Production