This rapid and simple specific enzymatic method is used for the simultaneous determination of lactose, sucrose and D-glucose. Free D-glucose in the sample is determined directly with GOPOD Reagent by conversion to a red coloured quinoneimine dye compound through the combined action of glucose oxidase and peroxidase. Sucrose is hydrolysed to D-glucose and D-fructose with b-fructosidase, and measured as D-glucose. Lactose is hydrolysed to D-glucose and D-galactose using β-galactosidase (lactase) and measured as D-glucose.
Size
100 tests
Concentration
n/a
Applications
This rapid and simple specific enzymatic method is used for the simultaneous determination of lactose, sucrose and Dglucose in flour mixtures as well as other foodstuffs, pharmaceuticals, cosmetics and biological samples.