Lactate Dehydrogenase Assay Kits provide both fluorescence and absorbance-based method for detecting either L-LDH or D-LDH in biological samples such as serum, plasma, urine, as well as in cell culture samples. In the enzyme coupled assay, LDH is proportionally related to the concentration of NADH that is specifically monitored by a fluorogenic NADH sensor. This assay kit is specific for L-LDH. The signal can easily read by an absorbance microplate reader at ~575 nm or at the absorbance ratio of A575nm/A605nm to increase assay sensitivity. With the L-Lactate Dehydrogenase Assay Kit, we were able to detect as little as 3mU/mL LLDH in a 100 μL reaction volume. It is robust, and can be readily adapted for a wide variety of applications that require the measurement of L-LDH.