MMP-14 Inhibitor Screening Kit provides a simple, fast and high-throughput adaptable method to screen/study/characterize potential MMP-14 inhibitors. The assay utilizes the ability of MMP-14 to cleave a synthetic MCA-based peptide substrate to release free MCA (7- Methoxycoumarin-4-acetic acid), which can be easily quantified using a fluorometer or fluorescence microplate reader at Ex/Em = 325/420 nm. In the presence of a MMP-14 specific inhibitor, the cleavage of the substrate is reduced/abolished, resulting in decrease or total loss of the MCA fluorescence.