In eukaryotic cells, Isocitrate Dehydrogenase (IDH1, IDH2 and IDH3) is an enzyme that catalyzes the decarboxylation of Isocitrate producing α-Ketoglutarate and CO2. Mutations in both isoforms of IDH (IDH1 and IDH2) are commonly found in human cancers.The Mutant Isocitrate Dehydrogenase (Mutant IDH) causes a ``gain-of-function'', which reduces its affinity for isocitrate and favors the conversion of α-ketoglutarate to D-2-Hydroxyglutarate. D-2-Hydroxyglutarate (D2HG) is present at low level in normal cells and tissues, but is significantly elevated in metabolic diseases and various cancers. D2HG functions as an ``oncometabolite'' promoting cellular transformation. Recent studies show that increased Mutant IDH activity is associated with various cancers; therefore, detection of Mutant IDH activity is important for diagnosis and developing therapeutic strategies (e.g. Mutant IDH inhibitors). Mutant IDH assay kit provides a quick and simple assay to monitor the Mutant IDH activity in biological samples. In the assay, Mutant IDH oxidizes NADPH into an NADP+, which decreases the absorbance at 340 nm. The assay is simple, sensitive, and can detect Mutant Isocitrate Dehydrogenase activity lower than 2 mU/ml in a variety of cancer samples.
Size
100 assays
Concentration
n/a
Applications
This assay has a detection sensitivity as low as 2 mU.