Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
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Description
Epitope: aa 472-658 of isoform N4 (see additional information). 1B1 recognizes all isoforms produced by alternative splicing except ShAdd (also known as hts-PC or N32/R2 [Uniprot ID Q02645-3]). By deduction, the 1B1 epitope is located in the region of N4 [Uniprot ID Q02645-1] common to all isoforms but absent in ShAdd, and corresponds to aa 472-658 of isoform N4. 1B1 recognizes only one fragment resulting from proteolysis of Ovhts (N4): Ovhts Fus is recognized but Ovhts-RC is not.
Immunogen
GST-adducin fusion protein expressed in E. coli: aa. 465-903 Of N4 isoform [Uniprot ID Q02645-1]
Other Names
add; Add; Add-hts; add-like; adducin; hu li tai shao; Ovhts; CG43443