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Description
Monoclonal 2A4 recognizes an epitope in the C-terminal half of rat Type I hexokinase. Crossreactivity with the Type II or Type III isozymes is thought to be unlikely. The epitope recognized is a conformational epitope, and immunoreactivity is affected by conformational changes in the Type I hexokinase, e.g., as a result of binding certain ligands [Smith and Wilson (1992) Arch. Biochem. Biophys. 292: 165-178]. The antibody is reactive with the native enzyme (ELISA) but not the denatured form of the enzyme and thus is not useful for immunoblots. The functional effects of this antibody inhibits rat Type I hexokinase activity to >95%, the effect being on V max with no significant effect of Kms for either glucose or ATP [Smith and Wilson (1992) Arch. Biochem. Biophys. 292: 165-178.