Sample Type: Serum, plasma and other biological fluids. Intended Use: For quantitative detection of LZM C in human serum, plasma and other biological fluids. Cell lysates and tissue homogenates, though not tested, may potentially be used as samples. Sensitivity: <5.625ng/ml Precision: Intra-Assay CV: <8% Inter-Assay CV: <10% Range: 9.375-600ng/ml Specificity: Human Test Principle: This BioAssay? kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with LZM C. During the reaction, LZM C in the sample or standard competes with a fixed amount of LZM C on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to LZM C. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solu