For the determination of Phenylalanine, Tyrosine & Tryptophan a sample preparation has to be performed. Therefore a precipitation solution, containing an internal standard, is added. After centrifugation the supernatant is injected into the HPLC system. The isocratic separation via HPLC at 30°C lasts 10 minutes. The chromatograms are recorded by a UV-detector. The quantification is performed with the delivered plasma calibrator; the concentration is calculated via the ``internal standard method'' by integration of the peak heights respectively areas. Sample Types: plasma, whole blood, filter spots. Time: 15 minutes ? The Eagle Biosciences Phenylalanine, Tyrosine & Tryptophan HPLC Assay kit makes it possible to determine phenylalanine, tyrosine and tryptophan in an easy, fast and precise method. The Phenylalanine, Tyrosine & Tryptophan HPLC Assay kit includes all reagents for preparation and separation of the samples with exception of the columns (IC4000rp) and the controls (IC4000ko). Both can be supplied by Eagle Biosciences. Besides the complete test kits it is possible to order all components separately. Please request our single component price list.