The Eagle Biosciences Interleukin 2 (IL-2) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for interleukin 2 (IL-2) has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any interleukin 2 (IL-2) present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for interleukin 2 (IL-2) is added to the wells and binds to the combination of capture antibody-IL-2 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of interleukin 2 (IL-2) present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven interleukin 2 (IL-2) standard dilutions and interleukin 2 (IL-2) sample concentration determined. Sample Types: serum, plasma, cell culture. Range: 31.25 - 2000 pg/ml. Sensitivity: 7 pg/ml. Time: 3.5 hours ? Rat Interleukin 2 (IL-2) ELISA Assay Kit