Many methods of Estrogens determination based on HPLC-UV detection show low selectivity if problematic matrices are applied. This method of content determination of Estrogens combines the high selectivity of immunoaffinity columns with its high potential to concentrate elute and of purification by HPLC column. The following instruction gives a optimized protocol for enrichment of very low contents in the pg/g range. In this case a mass detector might be best suited.Please notice, that this instruction focuses on the handling with the IAC column. The given apparatus and detection (e.g. HPLC-FLD system) might serve as example among other possibilities. Sensitivity: n/a. ?